Human Milk Oligosaccharides and the Association with Microbiota in Colostrum: a pilot study

Abstract HMOs (Human milk oligosaccharide) has an important impact on maternal and infant health. Colostrum samples of 70 breastfeeding women in China were collected and recorded clinical characteristics. The major oligosaccharides and microbiota were quantitated in colostrum. The concentration of fucosylated HMOs in primipara was higher than that of multipara (p= 0.030). The concentration of N-acetylated HMOs in vaginal delivery milk was less than that of cesarean (p= 0.038). N-acetylated HMOs of breastfeeding women were less than that of breast pump (p= 0.038). Meanwhile, the concentration of LNT was positively correlated with Lactobacillus (r= 0.250, p= 0.037). DS-LNT was negatively correlated with Staphylococcus (r= -0.240, p= 0.045). There was a positive correlation between Streptococcus and LNFPII (r= 0.314, p= 0.011), Streptococcus and 3-SL (r= 0.322, p= 0.009). In addition, there was a negative correlation between 2'-FL and 3-FL (r= -0.465, p= 0.001). There was a positive correlation between LNT and LNnT (r= 0.778, p= 0.001). Therefore, the concentration of HMOs is related to number of deliveries, delivery mode, lactation mode and perinatal antibiotic. The concentration of HMOs is related to Lactobacillus, Streptococcus and Streptococcus in colostrum. In addition, there are connections between different oligosaccharides in content. The study protocol was also registered in the ClinicalTrails.gov (ChiCTR2200064454) (Oct. 2022).