Unleashing the power of acetylacetone: Effective control of harmful cyanobacterial blooms with ecological safety

Harmful algal blooms resulting from eutrophication pose a severe threat to human health. Acetylacetone (AA) has emerged as a potential chemical for combatting cyanobacterial blooms, but its real-world application remains limited. In this study, we conducted a 42-day evaluation of AA's effectiveness in controlling blooms in river water, with a focus on the interplay between ecological community structure, organism functional traits, and water quality. At a concentration of 0.2 mM, AA effectively suppressed the growth of Cyanobacteria (88 %), Bacteroidia (49 %), and Alphaproteobacteria (52 %), while promoting the abundance of Gammaproteobacteria (5.0 times) and Actinobacteria (7.2 times) that are associated with the degradation of organic matter. Notably, after dosing of AA, the OD680 (0.07 +/- 0.02) and turbidity (8.6 +/- 2.1) remained at a satisfactory level. AA induced significant disruptions in two photosynthesis and two biosynthesis pathways (P < 0.05), while simultaneously enriching eight pathways of xenobiotics biodegradation and metabolism. This enrichment facilitated the reduction of organic pollutants and supported improved water quality. Importantly, AA treatment decreased the abundance of two macrolide-related antibiotic resistance genes (ARGs), ereA and vatE, while slightly increased the abundance of two aminoglycoside-related ARGs, aacA and strB. Overall, our findings establish AA as an efficient and durable algicide with favorable ecological safety. Moreover, this work contributes to the development of effective strategies for maintaining and restoring the health and resilience of aquatic ecosystems impacted by harmful algal blooms.