Synergistic Treatment of Crude Enzymes from Bacillus Sp . Strains to Boost Anaerobic Fermentation of Rice Straw

Waste carbon straw like rice straw (RS) could be consumed as an ideal substrate in anaerobic fermentation for biofuel production. However, this practice is obstructed by the existence of the lignin polymer in RS. Enzyme lysates from individual strains and enzyme lysate mixture from all seven strains were used in to remove the lignin of waste carbon straw (RS). The effect of enzyme lysate on removing lignin and increasing fermentation rate was measured. Maximum delignification of RS was obtained from enzyme mixture of all seven bacterial strain crude samples as compared to individual enzyme lysates of these bacterial strains. A selective lignin reduction of 46.7