Isolation, Characterization and Evaluation of Antioxidant and Anticancer Activities from Isolated Components of Ixora Chinensis Lam. Flowers

This research sought to isolate potent phytoconstituents from Ixora chinensis Lam. flowers and evaluate their usefulness to treat cancer, as well as reducing oxidative radicals. Several solvents were used in the extraction of the flowers, including petroleum ether, ethyl acetate, and ethanol. In this study, compounds were separated from extracts using column chromatography, and their properties were determined using 13C NMR, 1H, IR, and mass spectrometry. Compounds were tested for antioxidant activity in vitro and biochemical activity. Breast cancer (MCF-7) and colon cancer (CACO-2) cell lines were used to test the extract's anticancer potential. The extract was found to have anticancer activity. On the other hand, in vivo anticancer action was tested on animals by measuring the levels of ferritin and CEA in their bloodstream. Oleanolic acid (1), 5,7-dihyroxy-,3'4'-dimethoxy flavone from petroleum ether extract, (2), 3-acetoxy-olean-12-ene-30-oic acid (3) from ethyl acetate extract, and masticadienoic acid (4) were isolated from ethanolic extract of I. chinensis flowers, and these compounds were discovered for the first time in the flowers of this plant. Results exhibited that the separated compounds (1-4) shown considerable anticancer activity against both the MCF-7 and the CACO-2 cell lines. Following clinical research, it is possible that I. chinensis flower extract may be employed as an alternative medication therapy in the treatment of breast and colon cancer.