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Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system

Wei-Feng Guo,Dan-Dan Guo, Fen Li,Shen-Zhai Shang, Ting-Wan Li, Ying-Chao Tang, Man Jiang,Fu-Chun Xu,Wei Gao

Plant cell reports（2023）

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Abstract
Key message The extensive application of CRISPR in cotton was limited due to the labor-intensive transformation process. Thus, we here established a convenient method of CRISPR in cotton by CLCrV-mediated sgRNA delivery.
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Key words
efficient genome editing,crispr/cas9,cotton,virus-mediated
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