An Immunosensor for the Detection of Speckle-type Poz Domain Protein Based on Pioneering Jasmine-like Cu@l-asp Hybrid Nanoflowers and Palladium-platinum Nanoparticles.

Abstract In this paper, we first synthesis three-dimensional(3D) jasmine-like Cu@L-Asp inorganic-organic hybrid nanoflowers to load palladium-platinum nanoparticles as the signal enhancer in order to quantify intracellular speckle-type POZ domain protein (SPOP). Scanning electron microscope (SEM), fourier transform infrared (FT-IR), energy dispersive spectrometer (EDS), X-ray photoelectron spectroscopy (XPS) analysis was used to characterize the newly synthesized materials. The newly formed Cu@L-Asp/Pd-PtNPs can catalyze the decomposition of hydrogen peroxide and exhibit excellent catalytic performance. When different concentration of SPOP is captured by SPOP antibody linked to the surface of Cu@L-Asp/Pd-Pt NPs, the current signal decreases with the increase concentration of SPOP. After optimization, the SPOP immunosensor exhibited a good linear response over a concentration range from 0.1 pg mL-1-1 ng mL-1. The proposed sensor demonstrates good stability, acceptable reproducibility and excellent selectivity to the SPOP in the presence of possible interfering substances and has great potential application for detecting other intracellular macromolecular substances.