In Silico and Experimental Analyses of Long Non-coding RNA TMPO-AS1 Expression in Iranian Patients with Gastric Cancer
INTERNATIONAL JOURNAL OF CANCER MANAGEMENT(2023)
摘要
Background: In recent decades, many long non-coding RNAs (lncRNAs) have been reported to play a prominent role in tumori-genesis and the progression of human cancers, including gastric cancer (GC), a leading cause of cancer death in Iranian men and women. Studies have demonstrated that thymopoietin antisense transcript 1 (TMPO-AS1) was upregulated in different cancers by acting as an oncogenic lncRNA.Objectives: This study aimed to evaluate the expression of lncRNA TMPO-AS1 in Iranian patients with GC.Methods: In order to conduct the present study, 40 gastric tumor samples and 40 marginal noncancerous counterparts were col-lected. The characteristics of patients' samples were recorded, and the TMPO-AS1 expression levels were evaluated by qRT-PCR anal-ysis. The Cancer Genome Atlas (TCGA) data for TMPO-AS1 were used and analyzed through GEPIA and TANRIC online tools. Receiver operating characteristic (ROC) curve analysis was used to estimate the diagnostic value. Student t-test, one-way ANOVA, and chi-square test were accomplished via SPSS software.Results: Our data demonstrated that TMPO-AS1 was overexpressed in cancerous tissues compared to adjacent nonmalignant ones (P = 0.0076). None of the demographic and clinicopathological data were associated with TMPO-AS1 expression levels. The TCGA data demonstrated that TMPO-AS1 was upregulated in GC tissues in comparison to adjacent nonmalignant ones (P = 0.001). ROC curve analysis suggested that TMPO-AS1 expression levels could discriminate GC tumor tissues from normal ones (AUC = 0.699, P = 0.001).Conclusions: Altogether, in our study, we demonstrated that lncRNA TMPO-AS1 may be considered a biomarker in Iranian patients with GC. However, further investigations are required to confirm the potential application of this lncRNA in diagnosis, prognosis, and therapeutic applications of GC.
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关键词
Gastric Cancer,Gene Expression,LncRNA,TMPO-AS1,In Silico
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