Essential Oil of Lippia Alba Protects Against Ischemic-Reperfusion Acute Kidney Injury

Ischemia/reperfusion (I/R)-induced Acute kidney injury (AKI) is characterized by hypoxia and productio of reactive oxygen species (ROS), which could be prevented with antioxidant agents. The center dot In ser a highlight no lo ger than 85 cha acters essential oil of Lippia alba (EOLA) chemotype citral-limonene is rich in components with antioxidant activity. So, this center dot studyInsert aaims highto ight evaluateno thelonger than 85nephroprotective charactrs. effect of the EOLA on in vivo and in vitro models of renal I/R. Male Wistar rats were submitted to right nephrectomy, followed by ischemia by clamping the renal artery in the center dot left Insert kidney a highand a ight no reperfusion.longer thanAnimals 85 receivdcharacters. EOLA (200 mg/kg) or vehicle three days prior to I/R surgery. Blood and urine samples were obtained to evaluate creatinine, urea, uric acid, and creatinine clearance. The left kidney was collected for histological evaluations and analysis of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH). HK-2 cells were used to evaluate the effect of EOLA on I/R in vitro, using the MTT assay. Moreover, scanning electron microscopy (SEM) was performed to evaluate cell morphological alterations. The I/R resulted in biochemical parameter alterations, with EOLA reverting all these alterations. Histological evaluation showed that EOLA protected the morphological changes caused by I/R. Also, EOLA was able to reduce TBARS and increase GSH levels in renal tissue. In the tubular cells, the EOLA partially reversed the damage caused by I/R, which was observed through the SEM. The EOLA showed effect against I/R-induced AKI through pre-treatment, protecting biochemical and oxidative parameters in vivo and reversing tubular cell damage in vitro.