The Effects of Amyloid-beta on Metabolomic Profiles of Cardiomyocytes and Coronary Endothelial Cells

Background: An increasing number of experimental and clinical studies show a link between Alzheimer's disease and heart diseases such as heart failure, ischemic heart disease, and atrial fibrillation. However, the mechanisms underlying the potential role of amyloid-beta (A beta) in the pathogenesis of cardiac dysfunction in Alzheimer's disease remain unknown. We have recently shown the effects of A beta 1- 40 and A beta 1- 42 on cell viability and mitochondrial function in cardiomyocytes and coronary artery endothelial cells. Objective: In this study, we investigated the effects of A beta 1- 40 and A beta 1- 42 on the metabolism of cardiomyocytes and coronary artery endothelial cells. Methods: Gas chromatography-mass spectrometrywas used to analyze metabolomic profiles of cardiomyocytes and coronary artery endothelial cells treated with A beta 1- 40 and A beta 1-42. In addition, we determined mitochondrial respiration and lipid peroxidation in these cells. Results: We found that the metabolism of different amino acids was affected by A beta 1- 42 in each cell type, whereas the fatty acid metabolism is consistently disrupted in both types of cells. Lipid peroxidation was significantly increased, whereas mitochondrial respiration was reduced in both cell types in response to A beta 1-42. Conclusion: This study revealed the disruptive effects of A beta on lipid metabolism and mitochondria function in cardiac cells.