Plasmid-free CRISPR/Cas9 genome editing in Saccharomyces cerevisiae.
Bioscience, biotechnology, and biochemistry(2023)
摘要
The current CRISPR/Cas9 systems in the yeast Saccharomyces cerevisiae cannot be considered a non-genetic modification technology because it requires the introduction of Cas9 and sgRNA into yeast cells using plasmid expression systems. Our present study showed that the yeast genome can be edited without plasmid expression systems by using a commercially available protein transfection reagent and chemically modified sgRNAs.
更多查看译文
关键词
Saccharomyces cerevisiae
,CRISPR/Cas9 system,direct protein transfection,genome editing,non-genetic modification
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要