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Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase [version 2; peer review: 3 approved]

Ulrich Dobramysl,Tom Beneke,Jeremy Charles Mottram,Carolina Moura Costa Catta-Preta,Richard J. Wheeler,Eva Gluenz

Wellcome Open Research（2023）

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Abstract
We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
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Key words
Leishmania mexicana,Genome,Pilon polish,eng
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