The Tolerance of Grapevine Rootstocks to Copper Excess and to the Use of Calcium and Phosphorus to Mitigate Its Phytotoxicity

High soil copper (Cu) concentrations in vineyards can cause phytotoxicity to grapevine rootstocks. In order to mitigate toxicity, the use of grapevine rootstock genetic variation and the application of amendments are possible strategies. The aim of this study is to assess the tolerance of grapevine rootstocks to Cu excess and whether phosphorus (P) and calcium (Ca) can reduce phytotoxicity caused by Cu. Grapevine rootstock seedlings were produced from selected stakes: Paulsen 1103 ( Vitis berlandieri × Vitis rupestris ); SO4 ( Vitis berlandieri × Vitis riparia ); IAC 572 (( Vitis Riparia × Vitis rupestris ) × Vitis caribaea ); and Isabel ( Vitis labrusca ). Seedlings were grown in nutrition solution added with the following treatments: 0.3 µM Cu (control); 60 µM Cu; 60 µM Cu and 62 mg L −1 P; 60 µM Cu and 400 mg L −1 Ca. High Cu concentration caused phytotoxicity in all rootstocks, impairing their growth and decreasing nutrient concentration and photosynthetic activity. P and Ca addition had positive effect on the photosynthetic activity of all rootstocks, although it was not enough to revert growth to levels comparable with controls. Overall, based on the results, the application of P and Ca was not efficient in mitigating Cu phytotoxicity in grapevine plants grown in solution. Isabel was the most sensitive rootstock to Cu phytotoxicity, whereas Paulsen 1103 and SO4 presented more tolerance and can be used, together with other management strategies, in contaminated vineyard areas. Therefore, careful genotype rootstock selection for use in high Cu soils is important, while Ca and P are not efficient mitigators of Cu toxicity.