Genetic Detection and Phylogenetic Tree Study for SARS COV-2 (N Gene) In Iraq

Aim of the current work to discovered and evaluation the effect of SARS-CoV-2 in positive samples in terms of N-gene Material and method, techniques for covid-19 were created with the partial sequencing of the genome (N gene) in real-time reverse transcriptase-polymerase (RT-PCR) chain reaction. After SARS-CoV2 isolation, an RT-PCR diagnostic test was developed for nose and pharyngeal swabs. The FAM sequence's RNA-dependent RNA polymerase gene, as well as the SARS-E CoV-2's and N genes, were used in RT-PCR experiments (diagnostic kit). RT-PCR assays targeting positive samples. Result, the N-gene is further studied and amplified by PCR before being sequenced and recorded in gene bank for 10 results of this research, identifying the N-gene alone would mask more than 95% of positive cases going to follow viral nucleic acid screening of COVID-19 patients. As a result, tracking repositive COVID-19 patients can become more easy, reliable, and cost-effective. This conclusion must be confirmed in a larger society.