转cry1Ab/cry1Ac基因玉米Cry1Ab/Cry1Ac融合蛋白表达及对亚洲玉米螟的室内杀虫效果
Acta Entomologica Sinica(2020)
Abstract
[目的]室内抗螟性评价是转Bt基因抗虫玉米研发和安全性评价的重要环节.[方法]采用酶联免疫吸附测定法(ELISA)测定了转cry1Ab/cry1Ac基因玉米ZZM030心叶中Cry1Ab/Cry1Ac融合杀虫蛋白的表达量;采用室内生测法测定了分别取食转基因玉米ZZM030和非转基因玉米X249心叶后亚洲玉米螟Ostrinia furnacalis敏感品系ACB-BtS、Cry1Ab抗性品系ACB-AbR和Cry1Ac抗性品系ACB-AcR初孵幼虫的存活率.[结果]转基因抗虫玉米ZZM030 4叶期和8叶期心叶中Cry1Ab/Cry1Ac融合杀虫蛋白的表达量分别是10.62和2.94 μg/g FW.敏感品系亚洲玉米螟初孵幼虫取食转基因玉米ZZM030心叶2d的存活率仅为23.6%,4 d后存活率为0,而取食非转基因对照玉米X249心叶4d的存活率高达93.1%.Cry1Ab抗性品系和Cry1Ac抗性品系初孵幼虫取食转基因玉米ZZM030心叶6d后的存活率分别为11.1%和12.5%,而取食非转基因玉米X249心叶6d后的存活率分别为81.9%和77.8%.[结论]转cry1Ab/cry1Ac基因玉米ZZM030心叶中高表达的Cry1Ab/Cry1Ac融合蛋白对亚洲玉米螟初孵幼虫具有极高的杀虫效果.
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