Identification of Promoters Recognized by RNA Polymerase-σE Holoenzyme from Thermus thermophilus HB8

Thermus thermophilus σE, an extracytoplasmic function σ factor from the extremely thermophilic bacterium Thermus thermophilus HB8, bound to the RNA polymerase core enzyme and showed transcriptional activity. With the combination of in vitro transcription assay and GeneChip technology, we identified three promoters recognized by σE. The predicted consensus promoter sequence for σE is 5′-CA(A/T)(A/C)C(A/C)-N15-CCGTA-3′.