In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine Bacillus cereus Isolated from Saudi Arabia
METABOLITES(2022)
摘要
A strain of Bacillus cereus was isolated from the Saudi Red Sea coast and identified based on culture features, biochemical characteristics, and phylogenetic analysis of 16S rRNA sequences. EPSR3 was a major fraction of exopolysaccharides (EPS) containing no sulfate and had uronic acid (28.7%). The monosaccharide composition of these fractions is composed of glucose, galacturonic acid, and arabinose with a molar ratio of 2.0: 0.8: 1.0, respectively. EPSR3 was subjected to antioxidant, antitumor, and anti-inflammatory activities. The results revealed that the whole antioxidant activity was 90.4 +/- 1.6% at 1500 mu g/mL after 120 min. So, the IC50 value against DPPH radical found about 500 mu g/mL after 60 min. While using H2O2, the scavenging activity was 75.1 +/- 1.9% at 1500 mu g/mL after 60 min. The IC50 value against H2O2 radical found about 1500 mu g/mL after 15 min. EPSR3 anticytotoxic effect on the proliferation of (Bladder carcinoma cell line) (T-24), (human breast carcinoma cell line) (MCF-7), and (human prostate carcinoma cell line) (PC-3) cells. The calculated IC50 for cell line T-24 was 121 +/- 4.1 mu g/mL, while the IC50 for cell line MCF-7 was 55.7 +/- 2.3 mu g/mL, and PC-3 was 61.4 +/- 2.6 mu g/mL. Anti-inflammatory activity was determined for EPSR3 using different methods as Lipoxygenase (LOX) inhibitory assay gave IC50 12.9 +/- 1.3 mu g/mL. While cyclooxygenase (COX-2) inhibitory test showed 29.6 +/- 0.89 mu g /mL. EPSR3 showed potent inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci. The exposure times of EPSR3 for the complete inhibition of cell viability of methicillin resistant S. aureus was found to be 5% at 60 min. Membrane stabilization inhibitory gave 35.4 +/- 0.67 mu g/mL. EPSR3 has antitumor activity with a reasonable margin of safety. The antitumor activity of EPSR3 may be attributed to its content from uronic acids with potential for cellular antioxidant and anticancer functional properties.
更多查看译文
关键词
Antistaphylococci,MRSA,antioxidant,anti-inflammatory,antitumor,Exopolysaccharide,Bacillus cereus
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要