Callus Growth and Ion Composition in Response to Long-Term NaCl-induced Stress in Two Sugarcane (saccharum Sp.) Cultivars
International Journal for Biotechnology and Molecular Biology Research(2017)
摘要
In this work, the effect of different concentrations of NaCl on calli induced from two sugarcane cultivars NCo310 and CP59-73 was studied. Growth and ion concentrations (Na+, Cl-, K+ and Ca2+) of calli were determined after 1, 2 and 3 months of stress with the objective to understand the cellular mechanisms operating in salt stress tolerance and to determine the implication of inorganic fraction in salt tolerance in sugarcane cultivars. A negative effect of the NaCl concentration and the duration of stress exposure on the callus rate growth was observed in both cultivars and with more extent in CP59-73 cv. Results showed an increase in Na+ and Cl– and a decrease in K+ and Ca2+ concentrations after 1, 2 and 3 months of salt stress exposure. It also showed that resistant cv. NCo310 stressed calli accumulated less Na+ and retained more K+ and Ca2+ than CP59-73 calli. Cl- appeared to be involved in osmotic adjustment since the resistant cv. NCo310 stressed calli accumulated more Cl- than CP59-73 ones. These results suggested that the resistance to salinity in sugarcane is associated with a high K+, Ca2+ and Cl- concentrations and a low Na+ concentration within cells. Key words: sugarcane (Saccharum sp.), salt stress, ion uptake, callus growth, long-term stress exposure.
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要