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多指标正交试验法优选红参水提工艺研究

Asia-Pacific Traditional Medicine(2021)

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Abstract
目的:优选红参的水提工艺.方法:采用正交试验法,以人参皂苷Rg1、Rb1、Re含量和干膏得率的综合评分为评价指标,以浸泡时间、提取次数、提取时间和加水量4个因素设计L9 (34)正交试验,优选红参的最佳水提工艺,并进行验证.结果:红参最佳水提工艺为加8倍量水,浸泡30 min,提取3次,每次0.5h.验证试验的干膏得率均值为56.47%、人参皂苷Rg1的含量均值为0.1977%、人参皂苷Re的含量均值为0.1045%、人参皂苷Rb1的含量均值为0.2025%,各指标的RSD均小于3%.结论:优选的水提工艺稳定可行,可为红参的相关制剂研究提供依据.
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