Proteolysis Targeting Chimaeras Induce the Degradation of Epidermal Growth Factor Receptor in Lung Cancer

• The PROTAC degraders synthesized and assessed in this project were designed as follows: To characterize the EGFR PROTACs, we used: • HCC-827 and NCI H-3255 human lung carcinoma cell lines with mutant EGFR • Western Blot to assess protein levels of EGFR and downstream targets upon drug treatments • Cell Proliferation Assay to determine impact of PROTACs on lung cancer cell proliferation • Liquid Chromatography Mass Spectrometry to assess the specificity of the EGFR PROTACs