Tissue Factor (Tf) Regulates Cell Viability, Proliferation And Invasion Of Human Gastric Cancer

To investigate the effect of tissue factor (TF) on the biological behaviors of human gastric tumor tissues and cell line. The recombinant pcDNA3.1-antisense TF and pcDNA3.1-sense TF plasmids were constructed and transfected into SGC7901 cells with high TF expression or MKN45 cells with deficiency of TF, respectively. MTT, colony formation, FCM and transwell chamber methods were used to evaluate the effect of TF on cell viability, proliferation, cell cycle distribution and invasion abilities of gastric cancer cells. We first found an increased expression of TF in 24 pairs of human gastric cancer tissues. Functionally, the expression of TF was inhibited in SGC7901 cells transfected with the recombinant plasmid expressing antisense TF. Compared with parental cells and empty vector cells, the proliferation of TF-inhibited SGC7901 cells was significantly decreased (P<0.05). Proliferation index (PI) declined from 0.357 in parental cells to 0.286, and the invasion ability was decreased significantly (P<0.001). However, TF was overexpressed in MKN45 cells transfected with sense TF recombinant plasmid. Compared with parental cells and empty vector cells, the PI value was increased from 0.277 in parental cells to 0.366. Colony formation assay showed the consistent results. Moreover, the invasive ability of the TF-overexpressing MKN45 cells was significantly increased (P<0.001). TF may be a regulator of viability, proliferation and invasion of gastric cancer cells.