Cotyledon loss of Astragalus membranaceus hindered seedling establishment through mineral element reallocation and carbohydrate depletion.

Tissue loss of plants caused by herbivores is very common in nature. As the storage and first photosynthetic organ, the loss of cotyledon severely impacts dicot seedling establishment and the subsequent growth. However, it is still not clear how plants adjust their metabolic strategy in response to cotyledon loss. In this study, we employed ICP-OES, GC and LC-MS to examine the effects of cotyledon removal (RC1: remove one cotyledon, RC2: remove two cotyledon) on mineral element distribution and metabolite changes in a traditional Chinese herbal plant, Astragalus membranaceus. The results showed that cotyledon removal had a greater effect on shoot than root growth. Specifically, RC2 revealed a more serious impact on shoot growth than RC1. Microelement Mn and Na in shoot increased more in RC2 than RC1. Macroelement K and microelement B in root increased in RC2. The metabolite results in shoot showed that sugars related to galactose metabolism reduced while amino acids significantly increased in RC2. In root, sugars related to fructose and mannose metabolism decreased in both RC1 and RC2 while most flavonoids increased in RC2. It can be concluded that cotyledon removal triggered different metabolic strategies in both root and shoot. In shoot, more Mn was absorbed to improve the lowered photosynthetic efficiency. Meanwhile, increased Na may have promoted carbohydrate consumption and amino acid synthesis, thereby maintaining shoot growth. In root, K and B participation in cell division and expansion increased, as well as the delivery and metabolism of carbohydrates, to maintain root system growth.