Extraction, purification process for yersinia pestis natural F1 antigen
user-5fe1a78c4c775e6ec07359f9(2008)
摘要
The invention discloses an extraction and purification method for the natural F1 antigen of the Yersinia pestis, which adopts a proposal combining glass bead treatment, ammonium sulfate precipitation and molecular sieve filtration to successfully extract and purify natural F1 antigen with high purity from the Yersinia pestis cultures. The immunological detection and the animal protection experiment prove that the natural F1 antigen obtained by the method has higher protective immune function and detection sensitivity. If detecting the F1 monoclonal antibody of the same mouse ascites sample with the natural F1 antigen in the invention and the rF1 obtained from expression having the same concentration, the result indicates that the antibody titer detected by the natural F1 antigen is obviously higher than the detection result by the rF1. The invention can play an important role in the development of Sub-unit Vaccine of plague and the detection and diagnosis of plague (e.g. immunological detection, etc.), which has a broad application prospect.
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关键词
Yersinia pestis,Antigen,Antibody titer,Ammonium sulfate precipitation,Monoclonal antibody,Extraction Purification,Immune system,Microbiology,Chemistry,Purification methods
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