Purification of the human papillomavirus 16 L1 protein from E. coli SHuffle® T7
Vacunas(2020)
摘要
•HPV-16 L1-His protein represented ∼ 12% of total proteins.•It was purified to a purity of ∼ 90% by means of Ni2+ ion affinity chromatography.•9 mg de pentamers of cultured L1-His/L was obtained after renaturalisation.
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关键词
HPV,Escherichia coli,L1 protein,Purification,Inclusion body
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