Purification of the human papillomavirus 16 L1 protein from E. coli SHuffle® T7

S.M. Brito Molina, Y. Serrano Rivero,A. Falero Morejón,E. Pimienta Rodríguez,S. Rodríguez Salgueiro, O. Ancheta Niebla, K. Marro Domínguez

Vacunas（2020）

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摘要

•HPV-16 L1-His protein represented ∼ 12% of total proteins.•It was purified to a purity of ∼ 90% by means of Ni2+ ion affinity chromatography.•9 mg de pentamers of cultured L1-His/L was obtained after renaturalisation.

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HPV,Escherichia coli,L1 protein,Purification,Inclusion body

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Purification of the human papillomavirus 16 L1 protein from E. coli SHuffle® T7

Purification of the human papillomavirus 16 L1 protein from E. coli SHuffle® T7