E. Coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme
openalex(2020)
Abstract
Robust [NiFe] hydrogenase 1 (Hyd1) from Escherichia coli is shown to have non-native, H2-dependent activity for FMN and FAD reduction, and to function as a promising recycling system for FMNH2 supply to flavoenzymes for chemical synthesis, giving a total turnover number over 10 thousand when coupled with an Old Yellow Enzyme ene reductase.
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Key words
asymmetric catalysis,biocatalysis,cofactor recycling,ene-reductase,hydrogenation
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