Therapeutic potential of a copper complex loaded in pH-sensitive long circulating liposomes for colon cancer management

•In vitro evaluation of antitumor activity of Cu(II) complex, Cuphen, and study of its potential mechanisms of action.•Achievement of a pH-triggered Cuphen release from long circulating liposomes at tumor microenvironment.•Preclinical validation of a safe Cu(II) complex nanoformulation with therapeutic potential in a murine colon cancer model.•Cu complex nanoformulations emerge as an alternative and effective therapeutic strategy for CRC management.