SDF-1α/CXCR4轴通过诱导胰腺癌上皮-间充质转化促进肿瘤迁移和侵袭
Chinese Journal of Pathophysiology(2019)
Abstract
目的:探讨SDF-1α/CXCR4轴对胰腺癌细胞迁移和侵袭能力的影响及其作用机制.方法:应用RT-qPCR检测4种胰腺癌细胞株CXCR4 mRNA的表达.Transwell实验检测外源性SDF-1α及其受体CXCR4靶向抑制剂AMD3100对胰腺癌细胞迁移和侵袭能力的影响.MTS法检测外源性SDF-1α及AMD3100对胰腺癌细胞活力的影响.Western blot法检测外源性SDF-1α及AMD3100对胰腺癌细胞上皮-间充质转化 (EMT) 相关标志物表达的影响.结果: (1) 4种胰腺癌细胞株均不同程度地表达CXCR4 mRNA, 其中PANC-1细胞株表达量最高. (2) 外源性SDF-1α可增强PANC-1细胞的迁移和侵袭能力, 该作用可被AMD3100所阻断. (3) 外源性SDF-1α处理PANC-1细胞72 h可增强细胞活力, 该作用可被AMD3100阻断. (4) 外源性SDF-1α通过上调SNAIL和TWIST促使PANC-1细胞发生EMT, 该作用可被AMD3100所阻断.结论:SDF-1/CXCR4轴通过促进胰腺癌细胞发生EMT而促进肿瘤迁移和侵袭.
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Key words
Pancreatic cancer,SDF-1/CXCR4 axis,Epithelial-mesenchymal transition
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