多黏菌素B发酵培养基的优化
Chinese Journal of Pharmaceuticals（2014）
Abstract
本研究在摇瓶水平上优化了多黏芽孢杆菌(Bacillus polymyxa)的发酵培养基,以提高多黏菌素B的产量.采用单因素试验法和响应面法优化发酵培养基配方,并在7L发酵罐中进行验证.优化后的发酵培养基配方为全麦粉6.7％、玉米浆粉0.3％、葡萄糖0.5％、硫酸铵0.9％、磷酸二氢钾0.03％和碳酸钙0.03％.此条件下,多黏菌素B的摇瓶发酵产量为0.95 g/L,较优化前提高120.9％,7L发酵罐中的产量为1.06 g/L.
MoreKey words
polymyxin B,medium optimization,response surface methodology,fermentation
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