荧光原位杂交技术检测沙眼衣原体的可行性研究
Journal of Guangxi Medical University(2014)
Abstract
目的:探讨荧光原位杂交(FISH)技术检测沙眼衣原体(CT)的临床应用及其意义.方法:将超高倍多媒体显微仪检测法(MMDI)和荧光定量PCR结果一致作为判定衣原体是否感染的扩大金标准,取符合扩大金标准判定的宫颈棉拭子标本纳入FISH技术检测.结果:130例样本中MMDI和荧光定量PCR检测均为CT阳性的有52例,CT阴性的有78例.FISH技术检测阳性的46例,阴性84例.FISH技术检测CT的灵敏度为84.62%,特异度为97.44%,阳性预测值为95.65%,阴性预测值为90.48%.结论:成功构建了FISH技术检测沙眼衣原体的技术平台,该技术弥补了MMDI特异性低及PCR可视性不足的缺点.
MoreKey words
chlamydia trachomatis,FISH,PCR,multimedia microsopy diagnostic instrument
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