Integration of liquid chromatography mass spectrometry with a heavy peptide response curve accurately measures unprocessed C-terminal lysine during peptide mapping analysis of therapeutic antibodies in a single run
Journal of Pharmaceutical and Biomedical Analysis(2021)
Abstract
•Novel, simple, accurate and cost-effective quantification of mAb unprocessed C-terminal K.•Incorporates heavy standards for both native and modified peptides in a single LC–MS2 run.•Applies to IgG1, IgG2, IgG3 and IgG4 antibodies.•<10% error evaluated across different mass spectrometers and therapeutic antibodies.•Similar response curve approach can be developed for other PTMs.
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Key words
Post-translational modifications,C-terminal lysine,Mass spectrometry,Antibody,Heavy isotopic standard
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