Production Enhancement Of Bacterial Cellulose Nanofiber Using Local Komagataeibacter Xylinus Sb3.1 Under Static Conditions

Bacterial cellulose (BC) is a nanostructured material mainly produced by genus Gluconacetobacter displays unique physicochemical and mechanical properties therefore it has many potential applications in biomedical, biosensor, food and other industries. However, application of bacterial cellulose faced one of the main big problems in industry, viz., low productivity. Herein, this work was undertaken with a view to enhance the BC production using Komagataeibacter xylinus SB3.1 under static condition through study the effective culture parameters that played a vital role in nanofiber production. The results revealed the successful production of BC nanofibers using K. xylinus SB3.1 under static conditions as obviously indicated from the SEM image. FTIR and XRD affirmed both the chemical structure and crystallographic nature of cellulose I of the produced nanofibers. In the improvement effort, maximum yield of BC reached 6.54 g/L under optimal conditions including the use of mannitol and yeast extract as the sole source of carbon and nitrogen during an 8 day incubation period at 30 degrees C with 8% inoculum size which reveals to the increase in the nanofiber production 3.3 folded times than others with unoptimized condition under the same stationary conditions of growth. This also opens up the window for utilizing a new locally isolated K. xylinus SB3 in industrial manufacturing with potential features.