Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression
STAR protocols(2021)
Abstract
Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This protocol describes the design and preparation of an NP platform for mitochondria-specific gene de-livery (mito-NP). We use mito-NP in primary liver fibroblasts that are transplanted into mice. Mito-NP can be used to deliver various nucleic acid therapeutics and to treat mitochondria-regulated diseases.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).
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Key words
Gene Expression,Genetics,Molecular Biology
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