HoxU of Synechocystis sp. PCC 6803 Heterologous Expressed in E. Aerogenes

In this study, hydrogenase gene hoxU of the Cyanobacteria Synechocystis sp. PCC 6803 was cloned and heterologous expressed in Enterobacter aerogenes by using the pGEX-4T-2-cat vector, and obtain the combinant strain. The hydrogen yield of recombinant strain has increased compared with the wild type. The hydrogen yield of recombinant strain E. aerogenes/HoxU was promoted from 1.14 mol H2/mol glucose to 2.12 mol H2/mol glucose and the peak hydrogen producing rate reached 83.5 mL.L-1.h-1, which was higher than that of wild type.