Protein Profiling of Chickpea (cicer Arietinum L.) During Wilt Disease (fusarium Oxysporum F. Sp. Ciceri)

The proteomic study was carried out in 2DE gel electrophoresis to check differentially expressed proteins during chickpea-wilt interaction in resistance (WR315) and susceptible (JG62) chickpea genotypes.The result of this study showed that total 1070 and 1215 spots were detected in JG-62 and WR-315, respectively.Out of 1070 spots, 480 spots were present in control group while 590 spots were present in wilt inoculated susceptible plants.In resistant group 550 spots were found in control while 665 spots were found in wilt inoculated chickpea.The molecular masses of selected spots were identified with the range of 22.5 KDa to 104.4 KDa with pH range 4.00 to 6.76.Many spots were shown the significant level of differential expression in wilt resistant and susceptible chickpea plants.These spots were taken from match area of susceptible variety.Among the % vol. of each spot were identified and match with each other.Among the 12 spots matches during wilt infection majority shows down regulated as they occupy very low spot area.