Influence of Different Factors on Biofilm Formation of Listeria monocytogenes and the Regulation of cheY Gene

•Temperature has the greatest effect on Listeria monocytogenes biofilm formation.•Effects of different factors on biofilm formation are strain-dependent.•Motility and hydrophobicity are positively related to biofilm-forming capacity.•cheY gene positively regulates biofilm formation via motility and hydrophobicity.