Thin layer immunoassay; an economical approach to diagnose Helicobacter pylori Infection in gastroduodenal ulcer disease patients of Pakistan; a comparative analysis

is a causative agent of gastritis, gastroduodenal ulcers and gastric adenocarcinoma. Gastric patient’s serums were screened for infection by thin layer immunoassay. A polystyrene plate coated with sonicate whole cell antigen (10 μg/ml). Two fold-diluted patient’s serum was allowed to react at 37 °C, incubated at 60 °C for 1 min over water bath and recorded water condensation pattern for antibody. Gastric patient’s blood samples (62% male and 6% female) were tested positive for while agewise 15–25 years males (36%) and 65–75 years females (50%) showed highest number of infection. Thin layer immunoassay showed sensitivity (72–67%), specificity (100%), accuracy (94–69%) and κ value (0.493–0.357) in comparison with wELISA, sELISA and kELISA. We conclude thin layer immunoassay was reliable, low cost, quick, simple and clinically useful method for diagnosis in patients of Pakistan.