Screening for the Genetic Diversity among Local and Pure Breeds of Asiatic Chickens Using Blood Protein Polymorphisms by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

In this study, blood samples for protein analysis were collected from chicken of pure (boiler and layer) and local breeds for genotyping through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoregrams showed that there were similarities and differences in the molecular weight (MW) of the serum proteins among the accessions. A total of 14 bands, 9 were polymorphic and 6 were monomorphic. About 64.28 percent polymorphism was observed among the 12 accession during resolution of the proteins. Dendrogram developed using Jaccard’s similarity coefficient by using UPGMA based Shan cluster revealed that the accession were clearly separated from one another and range of genetic similarity among the four strains was 69 percent to 100 percent. Dendrogram classified the 12 chicken accessions into three groups. First group was comprised of 4 accessions from Aseel (AS), White Leghorn (WL), Red Rhode Island-2 (RDD) and Red Rhode Island-1 (RCC). Second group had five accessions: White Plymouth Rock-2 (BDD), White Plymouth Rock-1 (BCC), White Cornish-1(BAA), Barred Plymouth Rock (RBB) and White Cornish-2 (BBB). Third group is consisting of three accessions with two closely related to White Cornish (SK) and Kadaknath (KD) and one distantly Red Cornish (RAA). It can be concluded that blood serum protein found useful in taxonomic study.