Reversal Of Cisplatin Resistance In Ovarian Cancer Cells Mediated By Naringin-Induced Cox-2 Expression Through The Nf-Kappa B Signaling Pathway

Objective: To investigate the effect of naringin on COX-2 expression in SKOV3/DDP cell lines and the mechanisms of potential signaling pathways. Methods: The effect of naringin at different concentrations on the expression of NF-kappa B and COX-2 in SKOV3/DDP cell lines was detected by RT-PCR and Western blot, respectively; the effect of NF-kappa B on the expression of COX-2 mRNA was studied by targeted silencing and overexpression of NF-kappa B, and bidirectional adjustment of NF-kappa B expression in SKOV3/DDP cells. In SKOV3/DDP cells overexpressing NF-kappa B, naringin was added and cultured for 48 hours before the expression of COX-2 mRNA was detected. Results: RT-PCR and Western blot showed that the gene and protein expression of NF-kappa B and COX-2 in SKOV3/DDP cells gradually decreased with the increase of naringin concentration. After overexpression of NF-kappa B, the expression of COX-2 increased; on the other hand, the expression of COX-2 was down-regulated after NF-kappa B was silenced; the high expression of COX-2 induced by overexpression of NF-kappa B could be down-regulated by naringin. Conclusion: Naringin can inhibit the expression of NF-kappa B and COX-2 in SKOV3/DDP cells, and such inhibition may be related to the regulation of NF-kappa B signaling pathway, which may be one of the mechanisms involved in the reversal of cisplatin resistance in ovarian cancer cells by naringin.