Microfluidic single cell bioanalysis to measure drug uptake on single multidrug resistant cancer cell as enhanced by ginsenoside rg3

This paper is to demonstrate that the microfluidic-based single cell chip can be applicable to study micro-gram level amount of ginsenosides on daunorubicin uptake in multidrug resistant (MDR) leukemia cells. Pure ginseng compounds are very hard to obtain and can only be obtained at a trace amount (mini grams level). This small amount sometimes is not enough for traditional methods to evaluate the MDR effect of these compounds. Here, the microfluidic single cell analysis method was used to evaluate the MDR effect of several ginsenosides. In this paper, we tested two ginsenoside compounds: 20(S)-Ginsenoside Rg3 (Rg3-S) and 20(R)-Ginsenoside Rg3 (Rg3-R)and found that Rg3-S provided a stronger effect on enhancing the intracellular accumulation of anticancer drug in the single MDR cancer cells.. KeywordsCancer cell, Fluorescence, Ginsenosides, Microfluidics.