Reclassification of Brevibacterium frigoritolerans DSM 8801 T as Bacillus frigoritolerans comb. nov. Based on Genome Analysis

16S rRNA gene sequence analysis showed that the type strain Brevibacterium frigoritolerans DSM 8801 T had the highest similarity (99.7%) with the reference strain Bacillus simplex NBRC 15720 T , followed by Bacillus muralis DSM 16288 T (99.6%), Bacillus butanolivorans DSM 18926 T (99.5%), and Bacillus loiseleuriae FJAT-27997 T (97.9%). This relationship is confirmed by the phylogenetic analysis indicating that Bre. frigoritolerans DSM 8801 T fell in the genus Bacillus group and formed a clade with the closely related Bacillus species. Average nucleotide identity and in silico DNA–DNA hybridization ( is DDH) values between strain DSM 8801 T and the most closed reference strain Bac. simplex NBRC 15720 T were both much lower than species definition threshold values of 95% and 70%, respectively, which indicated strain DSM 8801 T should not be affiliated to one of the validly named Bacillus species. The percentage of conserved protein (POCP) values between the strain DSM 8801 T and the type strains of the above species were 80.7%, 69.2%, 72.2%, 53.6%, and 50.0%, respectively, higher than the genus definition threshold value of 50%. The main isoprenoid quinone of strain DSM 8801 T was MK-7, the main polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidyl ethanolamine (PE), and the major fatty acids were anteiso-C 15:0 and iso-C 15:0 . On the basis of the phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, Bre. frigoritolerans DSM 8801 T should belong to the genus Bacillus , and be proposed to reclassify as Bacillus frigoritolerans comb. nov., with the type strain DSM 8801 T (=ATCC 25097 T = CCUG 43489 T = CIP 67.20 T = JCM 11681 T ).