Preconcentration of Catecholamins on Hypercrosslinked Polystyrene and Their Determination by High-Performance Liquid Chromatography
Journal of analytical chemistry(2019)
摘要
The use of hypercrosslinked polystyrene for the group sorption preconcentration of catecholamines is proposed. The conditions for the preconcentration of noradrenaline, adrenaline, and dopamine are optimized: a microcolumn (10 × 6 mm), mass of adsorbent 0.03 g, 25 mL of solution (pH ~ 8.5), and transmission rate of the solution 1.0 mL/min. Compounds were desorbed with 1 mL of 6 M acetic acid and determined in the eluate by reversed-phase HPLC with an amperometric detector ( E = 0.8 V). Adsorption preconcentration ensures the reduction of the limits of detection for catecholamines by more than 20 times. The limits of detection were 0.7 ng/mL for dopamine and 1 ng/mL for noradrenaline and adrenaline. The procedure was applied to the analysis of model mixtures based on urine.
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关键词
adrenaline,noradrenaline,dopamine,solid phase extraction,hypercrosslinked polystyrene,HPLC
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