Measuring Nonspecific Protein-Protein Interactions by Dynamic Light Scattering.
Methods in molecular biology (Clifton, N.J.)(2019)
摘要
Dynamic light scattering has become a method of choice for measuring and quantifying weak, nonspecific protein-protein interactions due to its ease of use, minimal sample consumption, and amenability to high-throughput screening via plate readers. A procedure is given on how to prepare protein samples, carry out measurements by commonly used experimental setups including flow through systems, plate readers, and cuvettes, and analyze the correlation functions to obtain diffusion coefficient data. The chapter concludes by a theoretical section that derives and rationalizes the correlation between diffusion coefficient measurements and protein-protein interactions.
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关键词
Biopharmaceuticals,Osmometry,Protein aggregation,Protein crystallization,Second virial coefficients
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