A ratiometric flavone-based fluorescent probe for hypochlorous acid detection with large Stokes shift and long-wavelength emission

A ratiometric fluorescent probe, FPT, was designed and synthesized for the selective and sensitive detection of hypochlorous acid (HClO) based on a flavone derivative under a single wavelength excitation. The addition of HClO to the solution of FPT induced a distinct fluorescence color change from orange to green. Probe FPT was successfully used to detect and image intracellular HClO in living zebra fish in a ratiometric manner.