Evaluation on intrinsic quality of Wolfiporia cocos influenced by cultivation methods by a multi-step IR macro-fingerprinting technique

Wolfiporia cocos is a well-known traditional East-Asian medicinal fungus. In this study, four Wolfiporia cocos samples (FC-DM, RC-DM, FC-YN and SC-YN) of three cultivation methods harvest from Dabie Mountain area and Yunnan province were discriminated and identified by a multi-step IR macro-fingerprinting technique. The correlation coefficient of the three cultivation methods of Wolfiporia cocos are higher than 0.98 in the range of 450–2600 cm−1. According to retrieving the spectrum in Sadtler database, the main compositions of Wolfiporia cocos are laminarin polysaccharide. While Correlation coefficients of synchronous 2D-IR spectra of FC-DM and other Wolfiporia cocos samples were furtherly calculated, acquiring a quantitative discrimination result. The thermal sensitivity of polysaccharides of SC-YN is different from other samples in the range of 850–1370 cm−1. The difference in thermal sensitivity of CH (1402 and 1474 cm−1) and amino acid (1666 or 1651 cm−1) could also be considered as the key factors to discriminate them. The result proved that the 2DCOS-IR could discriminate Wolfiporia cocos of different cultivation methods.