Emticicia Aquatilis Sp Nov., Isolated from A Freshwater Sample

A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.14(T), was isolated from a freshwater sample near Donghaksa temple in Daejeon, South Korea. On the basis of the results of 16S rRNA gene sequence comparisons, THG-DN6.14(T) was found to be most closely related to Emticicia sediminis JBR12(T) (99.1% sequence similarity), Emticicia oligotrophica DSM 17448(T) (97.6 %), Emticicia aquatica HMF2925(T) (96.5 %), and Emticicia ginsengisoli Gsoil 085(T) (94.4 %). The DNA-DNA relatedness between THG-DN6.14(T) and its phylogenetically closest neighbours was below 65.0 %. The DNA G+C content was 43.3 mol%. The major polar lipids were found to be phosphatidylethanolamine, an unidentified glycolipid and an unidentified aminoglycolipid. The major fatty acids were identified as C-16 : 0, iso-C-15 : 0, iso-C-17 : 0 3-OH, and summed feature 3 (C-16 : (1 omega) 7c and/or C-16 : 1 omega 6c). The respiratory quinone was menaquinone MK-7. These data supported the affiliation of THG-DN6.14(T) to the genus Emticicia. THG-DN6.14(T) could be distinguished from related species of the genus Emticicia by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Emticicia aquatilis sp. nov. is proposed, with THG-DN6.14(T) (= KACC 18540(T) = CGMCC 1.15958(T)) as the type strain.