New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time

•A new Multiplex RT-qPCR methods to detect at least 14 subtypes of BCR-ABL fusion genes in one tube.•High sensitivity for clinical detection of BCR-ABL fusion genes.•The specificity comparable to the FISH method for clinical samples.•Benefit the precise diagnosis, targeted therapy and minimal residual disease monitoring.