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Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2 T , the second Intestinimonas species cultured from the human gut.

MICROBIOLOGYOPEN(2019)

Cited 12|Views24
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Abstract
Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono-genomics, it is strictly anaerobic, nonspore-forming, rod-shaped, with catalase- and oxidase-negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37 degrees C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein-coding genes and 62 RNA-coding genes. Strain GD2(T) significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal.
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Key words
anaerobe,butyrate,culturomics,new species,taxono-genomics
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