Through modulation of cardiac Ca handling, UCP2 affects cardiac electrophysiology and influences the susceptibility for Ca -mediated arrhythmias.
EXPERIMENTAL PHYSIOLOGY(2017)
Abstract
Uncoupling protein 2 (mitochondrial, proton carrier) (UCP2) belongs to a superfamily of mitochondrial ion transporters. Owing to its beneficial influence on production of reactive oxygen species, it is suggested to reduce cardiac ischaemia-reperfusion injury. Recent studies have uncovered its ability to regulate mitochondrial Ca2+ uptake and therefore to influence cardiac cytosolic Ca2+ handling, indicating compensatory pathways to avoid toxic Ca2+ overload in Ucp2 knockout (Ucp2(-/-)) mice. However, the specific mechanisms and their impact on cardiac electrophysiology remain speculative. Molecular analyses, whole-cell patch clamp in cardiomyocytes and ECG studies were performed in Ucp2(-/-) and wild-type (WT) control mice. Furthermore, to explore the impact on cardiac arrhythmogenicity, ECG monitoring was performed in basal conditions and during Ca2+-mediated stress using Bay K 8644. Although cardiac ryanodine receptor2, NCX1, L-type Ca2+ channel (LTCC) and SERCA2a expression were not altered, Ucp2(-/-) mice revealed major variations in cardiac electrophysiology. The LTCC current and APD(90) were decreased in Ucp2(-/-) mice, indicating compensatory mechanisms. Furthermore, in Ucp2(-/-) mice, an increased slope factor of action potential upstrokes and more hyperpolarized resting membrane potential were measured, suggesting variations in cardiac excitability. In agreement with alterations of cellular physiology in Ucp2(-/-) mice, reductions in PR and QRS as well as shortening of the QTc interval were noted in ECG recordings. Importantly, an increased incidence of cellular after-depolarizations and more pronounced susceptibility to Ca2+-mediated arrhythmias were observed. Furthermore, although expression of UCP3 was not different, levels of PRMT1 were significantly higher in Ucp2(-/-) mice. Our observations indicate compensatory mechanisms by which Ucp2(-/-) mice prevent toxic cytosolic Ca2+ overload. UCP2-dependent modulations have a major impact on cardiac electrophysiology and influence susceptibility to Ca2+-mediated ventricular arrhythmias.
MoreTranslated text
Key words
UCP2,arrhythmia,mitochondria,calcium,L-type calcium current,Heart
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined