Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3.
MOLECULAR BIOSYSTEMS(2016)
摘要
A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.
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关键词
ubiquitin proteins,chemoselective reactions,dual-labeling
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