Determination Of Estrogens In Drinking Water Using Hplc-Dad

DETERMINATION OF ESTROGENS IN DRINKING WATER USING HPLC-DAD. An analytical procedure for determination of estriol, 17 beta-estradiol, estrone and 17 alpha-ethinylestradiol in drinking water is presented. The method employs solid phase extraction (SPE) and sample dechlorination as cleanup procedures, followed by HPLC-DAD analysis. Validation was carried out using RE No. 899/2003 guidelines established by the Agencia Nacional de Vigilancia Sanitaria (National Agency of Sanitary Surveillance, Brazil), with some adaptations. The statistically evaluated results have shown that the method is selective, precise (0,06% to 19,40% CV) and accurate (91,52% to 109,41% average recoveries). The developed method was applied to the analysis of these contaminants in drinking water from Sao Luis, MA.