Investigations Concerning Possibilities of Diagnosis and Treatement in One Pesthole of Bacterial Haemorrhagic Septicemia in Carp (Cyprinus Carpio)

Investigations were done on 7 alive samples in agonic or death stage, taken from an extensive exploitation pond, in north-east Transylvanian zone, with an water surface of 0,2 ha and a total fish quantity of about 200 kg, second summer carp of an average weight of 300 g/individual. As a result of clinical, bacterioscopy and bacteriology and also necropsy laboratory exam it were put in evidence some aspects. So, clinical exam has revealed, by the presence of integument hemorrhagic ulcerous lesions, also the presence of some crustacaee ectoparasites, Argulus foliaceus species, which have made easier the disease spreading in whole effective. Necropsy exam puts in evidence, anatomy-clinic, the presence of integument hemorrhagic lesions under a point or diffuse form, but also ulcers and muscle – cutaneous necrosis. As a following of bacterioscopy exam of smears done from pathologic material taken in cutaneous lesions and colored by Gram method it was ascertained the presence of a bacterial polymorph flora, of bacillus and cocobacillus type, Gram negative. Bacteriologic exam (cultural), realized on ordinary culture mediums (sauce, water) and selective mediums (agar with blood) isolates in pure culture germs from Aeromonas kind, basis on morphological and cultural characters, which are framed in Aeromonas genus patterns described by speciality literature. As a following of biochemical characters exam, with the help of multitest API20E system it was established that Aeromonas spp. strains isolated, belong to hydrophila group 1 species, species which is responsible of bacterial hemorrhagic septicemia performing in carp. By testing the sensitiveness to antibodies and chemiotherapy in strains which have been found as diagnosis (diffusiometric method of antibiogram), it was ascertained that group 1 Aeromonas hydrophila strains, isolated from disease pesthole, were in decreasing order sensitive to : Eurofloxocin, Florfenicol (Floron) and Oxitetracycline and resistant to Ampiciline, Amoxicilin, Eritromicin.